Kurs bilgisi

Purpose of the Course

To enable students of molecular biology and genetics and plant science to learn many analytical methods that they will use throughout their working life.

Course Content

In this course, chemicals such as natural products, antioxidant substances, phenolic compounds that you often hear in daily life; we will learn about extractions, isolations and purifications from plants and then their analysis by different chromatographic methods.

Learning Outcomes of the Course

·         What is natural products?

·         What can be the active ingredients in natural products?

·         What is extraction? What are the extraction methods?

·         What are the differences between traditional extraction methods and modern techniques?

·         How is purification and isolation done after extraction?

·         By which methods are the extracts analyzed?

·         What are chromatographic methods?

·         Which product should be analyzed by which method?

·         After all the theoretical lessons, experiments were carried out on the subject covered every week. After the experiments, all the explanations were put into practice. To reinforce the topics after practice.

Education Methods

·   Working sheets

·   Laboratory studies

Assessment Methods

· Mid-term exam

· Final exam

· Lab quizes for every week

WEEKLY COURSE FLOW

1

Natural products

A lot of samples

What are the examples of used in daily lives?

2

Natural medicines

Overview of extraction

Introduction to liquid-liquid extraction system

3

Traditional extraction methods

Maceration

Percolation

Digestion

4

Infusion

Reflux

Tincture

Pressurized Liquid extraction (PLE)

5

Soxhlet extraction

Steam distillation

Hydro distillation

Supercritical Fluid Extraction (SFE)

6

SFE

Ultrasound assisted extraction

Microwave assisted extraction

Solid phase extraction (SPE)

7

Solid phase micro extraction

Pulse Electric Field extraction (PEF)

Enzyme assisted extraction

8

Midterm exam

9

Isolation and Purification

Introduction to chromatography

Purpose of chromatography

Mechanism of separation

Thin Layer Chromatography (TLC)

10

Thin Layer Chromatography (TLC)

Paper chromatography

High performance Thin Layer Chromatography (HP-TLC)

Column Chromatography

11

Column Chromatography

Principles of column chromatography and a lot of samples

High performance Liquid chromatography (HPLC)

12

High performance Liquid chromatography (HPLC)

Components of HPLC

Detectors

Pumps

Columns

A lot of chromatogram reviews

13

Gas chromatography (GC)

Detectors

Columns

A lot of chromatogram reviews

14

Gas chromatography (GC)

Structure Determination

Spectroscopy principles

Absorption Spectroscopy

Emission Spectroscopy

UV-Vis Spectroscopy, FTIR, and NMR

RESOURCES

Course Books

·    The Central Science (8th ed.) by Brown, LeMay, and Bursten.

·    Journals:

[1] Rasul M. G.,Extraction, Isolation and characterization of natural products from medicinal plants, International Journal of Basic Sciences and Applied Computing (IJBSAC), 2018, 6, 1-6. https://www.ijbsac.org/wp-content/uploads/papers/v2i6/F0076122618.pdf

[2] Patek K., Panchal N., Ingle P., Techniques adopted for extraction of natural products extraction methods: maceration, percolation, soxhlet extraction, turbo distillation, supercritical fluid extraction, International Journal of Advanced Research in Chemical Science (IJARCS), 2019, 4, 1-12. http://dx.doi.org/10.20431/2349-0403.0604001

[3] Zhang Q. W.,Lin L. G., Ye W. C., Techniques for extraction and isolation of natural products: A comprehensive review, Chin. Med., 2018, 13-20. https://doi.org/10.1186/s13020-018-0177-x

[4] Dean J. R., Extraction Techniques in analytical Sciences, Wiley, Newcastle, UK, 2009.

MATERIAL SHARING

Document

Lecture notes, articles and other helpful resources will be shared with students through online course information system.

Exam

Exams will be done in the classroom.

EVALUATION SYSTEM

In-Semester Activities

Order

 Percentage of Contribution

Mid-term exam

1

40

Final exam (%70 Final exam + %30 Lab quiz mean)

2

60

Total

100

Contribution of the Final Examination to the Overall Course Grade

60

Contribution of the In-Semester Activities  to the Overall Course Grade

40

Total

100